Synthetic Biology Lab

School of Life Science, USTC

   中文版

 

Publications

 

 

2024

4.Zhang Kehui, Jiang Ziyun, Li Xingjiang, Wang Dongmei, Hong Jiong* 2024. Enhancing simultaneous saccharification and co-fermentation of corncob by Kluyveromyces marxianus through overexpression of putative transcription regulator. Bioresource Technology, 399, 130627.[full text]

3.Boyang Xu, Wangwei Zhang, Eryong Zhao, Jiong Hong, Xiangsong Chen, Zhaojun Wei*, Xingjiang Li* 2024. Unveiling malic acid biorefinery: Comprehensive insights into feedstocks, microbial strains, and metabolic pathways. Bioresource Technology 394, 130265.[full text]

2.Zhou, H., Xu, S., Xu, B., Jiang, C., Zhao, E., Xu, Q., Hong, J., Li, X.*, 2024. Effect of Caproicibacterium lactatifermentans inoculation on the microbial succession and flavor formation of pit mud used in Chinese Baijiu fermentation. Food Research International 175, 113730. [full text]

1. Hu Shenglin, Xu Li*, Xie Canglin, Hong Jiong*, 2024. Structural Insights into the Catalytic Activity of Cyclobacterium marinum N-Acetylglucosamine Deacetylase. Journal of Agricultural and Food Chemistry 72, 783–793. [full text]

2023

2.Zhang Nini, Wang Feier, Nwamba, Marknoah Chinenye, Wang Dongmei*, Hong Jiong*. 2023. Enhancing tolerance of Kluyveromyces marxianus to lignocellulose-derived inhibitors and its ethanol production from corn cob via overexpression of a nitroreductase gene. Industrial Crops & Products, 203, 117136.[Full text]

1.Wang Lingya, Wang anran, Wang Dongmei*, Hong Jiong*. 2023. The novel properties of Kluyveromyces marxianus glucose sensor/receptor repressor pathway and the construction of glucose repression-released strains. Microbial Cell Factories, 22, 123.[Full text]

2022

3.Zhang J, Xu T, Wang X, Jing X, Zhang J, Hong J, et al. Lignocellulosic xylitol production from corncob using engineered Kluyveromyces marxianus. Frontiers in Bioengineering and Biotechnology 2022;10:1029203.

2.Yang Xiaoxue,Wang Dongmei,Hong Jiong*. 2022. Carotenoid production from nondetoxified xylose mother liquid or corncob hydrolysate with engineered Kluyveromyces marxianus. Bioresource Technology, 364, 128080.[Full text]

1. Zhiling Kuang, Ke Jiyuan, Jiong Hong, Zhongliang Zhu, Liwen Niu. 2022. Structural assembly of the nucleic-acid-binding Thp3-Csn12-Sem1 complex functioning in mRNA splicing. Nucleic Acids Research, 50(15), 8882-8897. [Full text]

2021

3.Nini Zhang, Yingying Shang, Feier Wang, Wang Dongmei*，Jiong Hong* (2021), Influence of prefoldin subunit 4 on the tolerance of Kluyveromyces marxianus to lignocellulosic biomass-derived inhibitors. Microbial Cell Factories 20, 224 . DOI:10.1186/s12934-021-01715-y

2.Shenglin Hu, Zhefan Wang, Dongmei Wang. Jichao Wang*. Jiong Hong* (2021),The development of a heterologous gene expression system in thermophilic fungus Thermoascus aurantiacus. 3Biotech, 11:414. DOI:10.1007/s13205-021-02963-w

1.Marknoah Chinenye Nwamba, Guojie Song, Fubao Sun*, Marie Rose Mukasekurua, Hongyan Ren, Qing Zhang,Tishuang Cao, Huaming Wang, Haiyan Sun, Jiong Hong (2021), Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse. Bioresource Technology, 338: 125505. DOI: 10.1016/j.biortech.2021.125505

2020

3.Rui Ding, Laipeng Luo, Ruixiang Han, Meiling Zhang, Tingting Li, Jihui Tang, Shenghai Huang*,Jiong Hong* (2021),Rapid production of a novel Al(III) dependent bioflocculant isolated from Raoultella ornithinolytica 160-1 and its application combined with inorganic salts.Frontiers in Microbiology,11:622365. doi:10.3389/fmicb.2020.622365

2.Dan Wu, Dongmei Wang* and Jiong Hong* (2020) Effect of a Novel Alpha/Beta Hydrolase Domain Protein on Tolerance of K. marxianus to Lignocellulosic Biomass Derived Inhibitors.Frontiers in Bioengineering and Biotechnology，8:844. doi: 10.3389/fbioe.2020.00844

1.王冬梅，洪泂，2020 在耐热的马克斯克鲁维酵母中构建微生物细胞工厂.生物学杂志，37（1），p1-10.（特约综述）[Full Text] [Full Text 2]

2019

1.Yan Hua; Jichao Wang; Yelin Zhu; Biao Zhang; Xin Kong; Wenjie Li; Dongmei Wang; Jiong Hong* 2019 Release of glucose repression on xylose utilization in Kluyveromyces marxianus to enhance glucose-xylose co-utilization and xylitol production from corncob hydrolysate. Microbial Cell Factories. 18：24.DOI: 10.1186/s12934-019-1068-2

2018

5.Xin Kong， Biao Zhang，Yan Hua， Yelin Zhu，Wenjie Li，Dongmei Wang, Jiong Hong* 2019 Efficient L-lactic acid production from corncob residue using metabolically engineered thermo-tolerant yeast.Bioresource Technology. 273， p220-230, DOI:10.1016/j.biortech.2018.11.018

4. Bangchun Wang, Laipeng Luo, Dongmei Wang, Rui Ding*, Jiong Hong* 2018 Efficient purification of a recombinant tag-free thermostable Kluyveromyces marxianus uricase by pH-induced self-cleavage of intein and expression in Escherichia coli.3 Biotech 8:400 [Full Text]

3.DongmeiWang, Jiong Hong* 2018. chapter 11: Expression of Cellulolytic Enzymes in Yeast. in: Fungal Cellulolytic Enzymes， Microbial Production and Application, (Ed.) Fang, Xu, Qu, Yinbo,springer Press, pp. 201-221.DOI:10.1007/978-981-13-0749-2_11

2. DongmeiWang, Dan Wu, Xiaoxue Yang, Jiong Hong* 2018.Transcriptomic analysis of thermotolerant yeast Kluyveromyces marxianus in multiple inhibitors tolerance. RSC Advance.8 ,14177–14192|,DOI: 10.1039/C8RA00335A [Full Text]

1. Shenglin Hu,Dongmei Wang, Jiong Hong* 2018. A Simple Method for Beta-glucosidase Immobilization and Its Application in Soybean Isoflavone Glycosides Hydrolysis. Biotechnology and Bioprocess Engineering. 23(1),p 39–48, DOI 10.1007/s12257-017-0434-3 [Full Text]

2017

3. Wen Wan, Min Lu, Dongmei Wang, Xiaolian Gao,Jiong Hong* 2017. High-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment.Scientific Reports. 7， 6119； DOI : 10.1038/s41598-017-06428-0 [Full Text]

2.Wenjie Li,Shouteng Xu,Biao Zhang,Yelin Zhu,Yan Hua,Xin Kong,Lianhong Sun,Jiong Hong* 2017. Directed evolution to improve the catalytic efficiency of urate oxidase from Bacillus subtilis. PLoS ONE. 12(5): e0177877. https://doi.org/10.1371/journal.pone.0177877 [Full Text].

1. Zhang Guorong,Lu Min, Wang Jichao, Wang Dongmei, Gao Xiaolian, Hong Jiong* 2017.Identification of hexose kinase genes in Kluyveromyces marxianus and thermo-tolerant one step producing glucose-free fructose strain construction. Scientific Reports. 7, 45104; doi: 10.1038/srep45104 [Full Text].

2016

5.Zhu, Y., Hua, Y., Zhang, B., Sun, L., Li, W., Kong, X., Hong, J*. 2017. Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD. Microbial Cell Factories, 16,2. DOI: 10.1186/s12934-016-0620-6.[Full Text]

4.Zhang, B., Zhu, Y., Zhang, J., Wang, D., Sun, L., Hong, J.* 2017. Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose. Bioresource Technology, Vol.224,p553-562, DOI: 10.1016/j.biortech.2016.11.110 [Full Text]

3. Wang, J., Zhang, T., Liu, R., Song, M., Wang, J., Hong, J., Chen, Q.*, Liu, H.* 2017. Recurring sequence-structure motifs in (betaalpha)8-barrel proteins and experimental optimization of a chimeric protein designed based on such motifs. Biochim Biophys Acta-proteins and proeomics, 1865 165–175.

2. Wan, W., Wang, D., Gao, X.,Hong J* 2017. chapter 17: Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA. in: Synthetic DNA: Methods and Protocols, (Ed.) R.A. Hughes,Humana Press, Vol. 1472, pp. 217-235.

1. Zhang B, Zhang J, Wang D,Hang R, Ding R, Gao X, Sun L, and Hong J* (2016)Simultaneous fermentation of glucose and xylose at elevated temperatures co-produces ethanol and xylitol through overexpression of a xylose-specific transporter in engineered Kluyveromyces marxianus. Bioresource Technology,216：p227-237.[Full Text] DOI: 10.1016/j.biortech.2016.05.068 （Best article in this issue)

2015

4. Zhang B, Zhang J, Wang D, Gao X, Sun L, and Hong J*, Data for rapid ethanol production at elevated temperatures by engineered thermotolerant Kluyveromyces marxianus via the NADP(H)-preferring xylose reductase–xylitol dehydrogenase pathway. Data in Brief, 2015. 5: p. 179-186.[Full Text]

3.Zhang J, Zhang B, Wang D, Gao X, Sun L, and Hong J* (2015) Rapid ethanol production at elevated temperature by engineered thermo-tolerant Kluyveromyces marxianus with NADP(H)-preferring xylose reductase-xylitol dehydrogenase pathway. Metabolic Engineering. 31: p. 140-152.[Full Text]

2. Yang C, Hu S, Zhu S, Wang D, Gao X, and Hong J* (2015) Characterizing yeast promoters used in Kluyveromyces marxianus. World Journal of Microbiology and Biotechnology.31: p. 1641-1646.

1.Hu D, Hu S, Wan W, Xu M, Du R, Zhao W, Gao X, Liu J, Liu H*, and Hong J* (2015) Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies. PLoS One. 10(6): p. e0129125.

2014

6.Man Xu, Siyi Hu, Bo Ding, Caiyi Fei, Wen Wan, Dongmei Hu, Ruikai Du, Xiaochuan Zhou, Jiong Hong, Haiyan Liu, Xiaolian Gao*, Jing Liu* (2015). Design and construction of small perturbation mutagenesis libraries for antibody affinity maturation using massive microchip-synthesized oligonucleotides. Journal of Biotechnology, 194, 27–36.[Full Text]

5.Zhang, Jia, Zhang Biao, Wang Dongmei, Gao Xiaolian, Hong Jiong* (2015). Improving xylitol production at elevated temperature with engineered Kluyveromyces marxianus through over-expressing transporters. Bioresource Technology, 175, 642-645. DOI: 10.1016/j.biortech.2014.10.150.[Full Text]

4. Wang Dongmei, Hong Jiong* (2014) Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag. Methods Mol Biol 1177:35-45. doi:10.1007/978-1-4939-1034-2_3 [Full Text]

3. 葛永斌, 洪泂, 王冬梅* (2014) 合成生物学中的正交遗传系统. 生命的化学 34 (3):376-384.[Full Text]

2. WAN, Wen; LI, Lulu; XU, Qianqian; WANG, Zhefan; YAO, Yuan; WANG, Rongliang; ZHANG, Jia; Liu, haiyan; Gao, Xiaolian*; Hong, Jiong* (2014) Error removal in microchip-synthesized DNA using immobilized MutS. Nucleic Acids Research,42(12),e102, doi: 10.1093/nar/gku405 [Free Full Text]

1. Wang Rongliang, Wang Dongmei, Gao Xiaolian, Jiong Hong* (2014) Direct fermentation of raw starch using a Kluyveromyces marxianus strain that expresses glucoamylase and alpha-amylase to produce ethanol. Biotechnology Progress 30(2): p. 338-347, DOI:?10.1002/btpr.1877.[Full Text]

2013

3.Jia Zhang, Bio Zhang, Dongmei Wang, Xiaolian Gao, Jiong Hong* Xylitol production at high temperature by engineered Kluyveromyces marxianus. Bioresource Technology 2014 152:192-201 [Pubmed] [Full Text]

2.Rongliang Wang, Lulu Li, Biao Zhang, Xiaolian Gao, Dongmei Wang*, Jiong Hong* Improved xylose fermentation of Kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway.Journal Of Industrial Microbiology & Biotechnology2013 40 (8):841-854.[Full Text]

1.Zhang Biao, Li Lulu, Zhang Jia, Gao Xiaolian,Dongmei Wang*, Jiong Hong*,Improving ethanol and xylitol fermentation at elevated temperature through substitution of xylose reductase in Kluyveromyces marxianus. Journal Of Industrial Microbiology & Biotechnology 2013,40 (3-4):305-316. doi:10.1007/s10295-013-1230-5 [Full Text]

2012

2. 王冬梅，宛雯，洪泂* DNA化学合成中的错误去除， 生命的化学， 2012 32(1)34-38[Full Text]

1. Lulu Li,Ling Zhang, Dongmei Wang, Hisanori Tamaki, Hidehiko Kumagai, Xiaolian Gao, Jiong Hong*, Identification of A Xylitol Dehydrogenase Gene from Kluyveromyces marxianus NBRC1777. Molecular Biotechnology 14 Feb 2012 online first DOI: 10.1007/s12033-012-9508-9[Full Text]

2011

5.Wen Wan, Dongmei Wang, Xiaolian Gao and Jiong Hong*，Expression of family 3 cellulose-binding module (CBM3) as an affinity tag for recombinant proteins in yeast.Applied Microbiology and Biotechnology 2011 V91(3):789-798[Pubmed] [Full Text]

4.Biao Zhang, Ling Zhang, Dongmei Wang, Xiaolian Gao and Jiong Hong*，Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777.Journal Of Industrial Microbiology & Biotechnology 2011 V38(12)2001-2010 [Pubmed] [Full Text]

3. Deguo Xia,Haoran Zheng,Zhiqiang Liu, Guisheng Li, Jinlong Li, Jiong Hong and Kai Zhao,MRSD: a web server for Metabolic Route Search and Design, Bioinformatics. 2011 27(11): 1581-1582 [Pubmed] [Full Text]

2. Rongliang Wang, Ling Zhang, Dongmei Wang, Xiaolian Gao and Jiong Hong*，Identification of a xylulokinase catalyzing xylulose phosphorylation in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777.Journal Of Industrial Microbiology & Biotechnology. 2011 V38(10)1739-1746 [Pubmed] [Full Text]

1. 王冬梅 洪泂*, 从碱基到人造生命——基因组的从头合成, 生命的化学 2011，Vol31（1），13-20. [摘要] [全文]

 

Before 2010

  1. Jiong Hong, Ye Xinhao, Wang Yiran, Zhang Y-HP. Bioseparation of recombinant cellulose binding module-protein by affinity adsorption on an ultra-high-capacity cellulosic adsorbent. Analytica Chimica Acta 2008,Vol 621,193-199.
  2. Jiong Hong, Yiran Wang, Xinhao Ye, Y-H Percival Zhang, Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage,Journal of Chromatography A 2008,Vol 1194,150C154.
  3. Jiong Hong, Xinhao Ye, Y-H Percival Zhang, Quantitative Determination of Cellulose Accessibility to Cellulase Based on Adsorption of a Non-Hydrolytic Fusion Protein Containing CBM and GFP with Its Applications,Langumir 2007,Vol 23,12535-12540.
  4. Jiong Hong, Yonghong Wang, Hisanori Tamaki, and Hidehiko Kumagai, Construction of thermotolerant yeast expressing thermostable cellulase genes, Journal of Biotechnology 2007 Vol. 130,114-123. [Full Text]
  5. Jiong Hong, Hisanori Tamaki, and Hidehiko Kumagai,Cloning and functional expression of thermostable b-glucosidase gene fromThermoascus aurantiacus, Applied Microbiology and Biotechnology 2007,Vol. 73,1331-1339.
  6. Jiong Hong, Hisanori Tamaki, and Hidehiko Kumagai, Unusual hydrophobic linker region of bglucosidase (BGLII) from Thermoascus aurantiacus are required for hyper activation by organic solvents. Applied Microbiology and Biotechnology 2006,Vol. 73,80-88.
  7. Zhang YH, Hong J, Ye X. Cellulase assays.Methods Mol Biol. 2009;581:213-31.
  8. Liu W, Hong J, Bevan DR, Zhang YH. Fast Identification of Thermostable Beta-Glucosidase Mutants on Cellobiose by a Novel Combinatorial Selection/Screening Approach. Biotechnology and Bioengineering, 2009. 103(6):1087-1094.
  9. Hong Jiong, Tamaki Hisanori, Akiba Shunichi, Yamamoto Kenj.Kumagai Hidehiko, Cloning of a gene encoding a highly stable endo-beta-1,4-glucanase from Aspergillus niger and its expression in yeast. Journal of Bioscience and Bioengineering, 2001. 92(5):434-441.